I would like to manipulate the expression of a gene in the dentate gyrus during the first 2 weeks of postnatal life via vector delivery. I am in need of a DG-specific promoter that has a non-fluctuating expression during that period.
Thank you so much for responding to my question.
In mice, there are several promoters that have been shown to exhibit dentate gyrus (DG)-specific expression during neurodevelopment. One commonly used promoter is the Dentate Gyrus-Selective promoter (DGNP-1). DGNP-1 has been found to drive gene expression specifically in the DG of the hippocampus during development.
Another DG-specific promoter that has been utilized is the Prox1 promoter. Prox1 is a transcription factor that is highly expressed in the DG granule cells. Its promoter has been shown to drive gene expression specifically in the DG.
Additionally, the Nestin promoter has been used to target gene expression to neural stem cells in the DG during development. Although Nestin is not exclusive to the DG, it can be used to manipulate gene expression in the DG neurogenic niche.
These are just a few examples of DG-specific promoters that have been used in mouse models to manipulate gene expression during neurodevelopment. It's important to note that the choice of promoter may depend on the specific gene or genes you are interested in manipulating and the experimental design you have in mind. It's recommended to consult the literature and seek expert advice to determine the most appropriate promoter for your research goals.
Thank you for answering my question Dr. Falakdin. Would you mind sharing a reference for the Dentate Gyrus-Selective promoter (DGNP-1)?
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