We use in our system Acclaim PepMap100 C18 for the pre-concentration, which ususally has very high backpressure in the last months. Did somebody have the same problem?
I red that in some of the thesis, in which they used this column in back-flush mode? Is that feasible?
System: UltiMate™3000 RSLCnano
Pre-column: Acclaim PepMap100 C18 5 μm, 100 Å, 100 μm×2 cm, ThermoFisher Scientific
I can suggest using the trap columns at reverse flow elution mode in place of forward elute approach if the your system configuration is convenient. This will reduce peak broadening and clogging. Indeed the sampel prep and loading amounts are the most critical parameters to keep the column backpressure stable over a relatively long period. Besides, monolithic C18 nanocolumns are also available they are more robust against dirty matrices and can be used to enrich peptide digest prior to analysis.
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