I wonder if THF, on its own, has any transesterificaiton potential.
I need to seperate an ester conjugate from its acid form by HPLC. Mobile Phase contains ACN:THF:Water. The problem is that I see a huge peak exactly on the retention time of the acid form when I enject the conjugate.
Not likely. The protons are not significantly available for protonation like Methanol or Water. You are more likely seeing an adduct or contamination from the column or HPLC system.
I recommend Prof. Bruce answer. But what if, you don't use THF in your mobile phase. There are many other solvents to maintain same polarity as with THF. Try it.
Thanks
Water is the strongest nucleophile in your mobile phase and may cause the hydrolysis, particularly if you did not neutralize your sample. As already mentioned, THF should not be the problem.
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