I would like to do Luciferase reporter assay to identify regulatory sequences/sites in the promoter region of my gene of interest (both in human and mouse). I am aware that this experiment involves the construction of reporter plasmids that contain the gene regulatory element fused to a core promoter upstream of a reporter gene like luciferase. Can anyone suggests me a company which constructs these kinds of reporter plasmids or any other ideas are welcome.
Addgene is a reliable source to start off at. As the plasmids are validated and have been published. Genecopecia and switchgear genomics are additional two websites u could visit. They have a Database of reporter plasmids as well provisions for having them customised.
SGI DNA can synthesize any custom plasmids for you. So you can even ask them to add the promoter sequence you are trying to verify. Its very cost effective.
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