Why wont my microbial fuel cell generate any current?

I have constructed a 2 chamber MFC for my senior research assignment and it has yet to produce any voltage. I used nafion as a PEM membrane and used graphite brushes as the electrodes. I have the...

11 January 2021 5,553 4 View

NAND flash recovery?

Hi Due to not being able to access University tools etc. I am stuck using recovery software such as recuva etc. I guess the question I'm trying to ask is how would I go about analysing results...

12 October 2020 437 1 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

Contrasting singular means?

Does anyone know of a method or approach in SPSS to contrast singular mean scores of overall samples, based only on the overall mean value, standard deviation figure and sample size? The data is...

29 September 2020 5,872 6 View

No title

Due to COVID- 19, pupil teacher of teacher training programs are doing school internship in online mode which they are completely opposed to. What do you think as to it will adversely affect their...

09 August 2020 756 20 View

How to correct invalid atom type error in gromacs?

27 April 2020 1,318 3 View

How to fix lincs warning in free energy calculation?

Dear Researcher I am having trouble during free energy calculation in gromacs as below:- I ran an MD simulation for 3ns and from the last coordinates started a free energy calculation for 3ns. My...

24 March 2020 5,311 9 View

How to visualize CHGCAR file of VASP in VESTA?

In vesta, I am not able to visualize CHGCAR file created by vasp. As I try to open it, the vesta window turns grey. kindly help

23 March 2020 1,155 7 View

Why peak current of oxidation or reduction of analyte decreases in MOF modified electrodes compared to bare?

I am working on Metal Organic Frameworks for electrochemical sensing of organic pollutants. For this, I am preparing MOF solution (1mg/ml ethanol) and dropcast on GCE, air dry then drop cast...

27 February 2020 525 4 View

How to fix Residue mapping error for an amino acid in gromacs?

Dear Gromacs users I want to do MD simulation for my protein but when I run pdb2gmx command I am facing this error:- WARNING: WARNING: Residue 197 named PRO of a molecule in the input file was...

12 November 2019 5,425 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View