Thanks.
thanks Tom Tomian.. but sadly i dont have the florescence detector in my lab.
You could determine via electrochemistry using pt. http://www.pnas.org/content/109/29/11534.full.pdf+html
Ive seen papers in which people measure OH by the extent / rate of hydroxylation of benzoic acid.
In your sample containing Oxygen it contains un paired electrons it gives ESR spectra.The free radicals can be easily identified by using ESR spectroscopy. It gives bands of ESR signals.
Thank you all for your worthy suggestions
PL is a very effective method. EPR is too expensive and not available for anybody.
i am unable to interpret why its increases in start as shown in figure
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