Better loading control (Western blot) for neurodegenerative disease brain tissue?

I am performing western blot analysis to detect the protein expression changes in the brain tissues from several neurodegenerative diseases (e.g Alzheimer's, Parkinson's) as compared to normal...

05 June 2018 549 8 View

Does anyone have protocol to measure lipid peroxidation/MDA levels in the brain tissue using 96 well plate?

Would like to measure oxidative stress in the brain tissue. I am thinking to measure lipid peroxidation using MDA levels. If anyone has any suggestions, to measure oxidative stress in the brain...

31 December 2014 9,009 7 View

Is it possible that one can detect desired protein using western blot but not using immunocytochemistry/immunohistochemistry?

One of the research groups has shown that they are not able to detect ERK5 protein expression (taking into consideration proper negative and positive control) in brain using immunohistochemistry...

05 June 2013 9,530 28 View

If one wanted to determine whether particular growth factor/neurotrophic factor activated desired protein, what would be the ideal treatment protocol?

A) Serum withdrawal prior to treatment / lower the serum concentration from 10% to 0.5 or 2.5% OR B) No serum withdrawal at all prior to treatment and perform treatment in regular media.

04 May 2012 6,373 5 View

What is the best stripping solution recipe (in house) and method for Nitrocellulose and PVDF membrane?

04 May 2012 7,278 53 View

Which is better for the washing step after primary antibody or secondary antibody treatment - 1X TBST or 1X PBST solution?

A) Why would one prefer 1XTBST over 1XPBST or vice versa? B) If one is detecting phospho proteins, does it matter which one you are using? I have been doing Western Blots for a very long time...

04 May 2012 1,693 14 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View