Is the pancreatic tissue still sensitive to pharmacological treatment after freezing in O.C.T. ?

More Andrei Alexandru CONSTANTINESCU's questions See All

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Soft agar experiment: I noticed overnight after plating upper layer, agar becomes thinner, almost liquid. Is not normal or not? How to prevent it?

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Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

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Claudio Sorio

I am not expecting that you are dealing with live tissue any more. If you want to cryopreserve pancreatic cancer tissue please refer to our work we published back in 2001 (see attachment). Hope this helps too. Claudio

Article Successful xenografting of cryopreserved primary pancreatic cancers

Matthias Löhr

If you thaw now the frozen-down entire "normal" pancreatic gland, then the proteases will chew up the tissue. The immunosuppressors will not work on tissue slices - unless you cut "thick/organotypic" ones Even then, the out one is unclear to be optimistic.