Is the expression of housekeeping genes always constant?

08 August 2018 0 9K Report

Is the gene expression of the housekeeping genes (such as 16S rRNA gene) cannot be affected by experimental factors? and this means the level of expression is always constant (the same CT value).

Badges
Science topic

More Kais Kassim's questions See All

Why the size of amplicons 80–200 bp are acceptable in real-time PCR?

Hi, can i ask if using more than 200 bp of amplicons in real-time PCR may give not good results ?

10 November 2016 3,285 12 View

What is the best abiotic surface for biofilm formation by bacteria ?

Can I ask about the perfect abiotic surface which give good results for biofilm formation by using gram negative bacteria when we study the effect of other factors such as temperature,...

09 October 2016 7,503 0 View

I have a question about the evaluation of Delta Delta Ct method for measurement the gene expression ? and is this method need any statistics ?

my work on blaOXA genes in Acinetobacter and i want to use the method Delta Delta Ct for measurement the gene expression by using quantitative RT PCR assay , is this method give Certified results...

05 June 2016 7,666 5 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,514 0 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View