Kais Kassim

4 Questions 21 Answers 0 Followers

Questions related from Kais Kassim

Is the expression of housekeeping genes always constant?

Is the gene expression of the housekeeping genes (such as 16S rRNA gene) cannot be affected by experimental factors? and this means the level of expression is always constant (the same CT value).

08 August 2018 9,002 0 View

Why the size of amplicons 80–200 bp are acceptable in real-time PCR?

Hi, can i ask if using more than 200 bp of amplicons in real-time PCR may give not good results ?

11 November 2016 3,228 12 View

What is the best abiotic surface for biofilm formation by bacteria ?

Can I ask about the perfect abiotic surface which give good results for biofilm formation by using gram negative bacteria when we study the effect of other factors such as temperature,...

10 October 2016 7,460 0 View

I have a question about the evaluation of Delta Delta Ct method for measurement the gene expression ? and is this method need any statistics ?

my work on blaOXA genes in Acinetobacter and i want to use the method Delta Delta Ct for measurement the gene expression by using quantitative RT PCR assay , is this method give Certified results...

06 June 2016 7,625 5 View