I have exposed microalgae cells to temperature stress and measure the ROS level using DCFH, but I found that the DCFH was significantly lower in stress-treated samples compared to control. How is this possible?
Dear Bahram Barati,
There are chances of decreased expression of ROS in the system under the stress condition if this is the result of the repeated standardized experiment.
But, decreased expression of ROS also tagged with other parameters such as involvement of various antioxidant enzymes, the incubation time factor, methodology of the experiment, etc.,
you can run parallel experiments regarding the antioxidant enzyme studies and GSH in order to confirm with the obtained result (decreased ROS in stress condition than Control).
All the best
I assume even if the ROS scavenging enzymes are activated they will maintain the ROS level as much as the control, not lowering it to below the control.
Yes, because zero levels of ROS will never occur. Taking into account that these species will always be produced by cellular metabolism, and their balance in the cell is of vital importance for the cell maintenance process.
Generally, the elevation of ROS always occurs preceded by a cellular imbalance in response to some pathogenic attack, infectious processes or even changes in factors in the conditions of the cellular environment. Then, your levels will only decrease when these conditions are extinguished.
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