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I have exposed microalgae cells to temperature stress and measure the ROS level using DCFH, but I found that the DCFH was significantly lower in stress-treated samples compared to control. How is...
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One of the useful applications of RNAi technology has been discussed as a therapy. Based on the current state of this technology is this therapy methd currently used for humans? Is it more...
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There are many ideas to create genetically modified microalgae for higher productivity, Why are the reports on this so little? I think now it is more possible with CRISPR technology. Any idea of...
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algae have been used in wide range of industries from being as food nutrient to bio-plastic. What is the most practical uses (commercially) of algae?
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I have measured ROS levels of Chlorella using DCFH before and after exposed to heat stress with following intervals 3h, 6h, 9h, 24h and 48h. The level of ROS before the treatment was higher. How...
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I am going to study the expression level of several target genes using qPCR. In my experiment, I expose microalgae culture to heat stress. then after I harvest the culture in different time points...
06 April 2017 8,547 6 View
I am going to study the expression of 8 target genes in respond to heat stress using qPCR. For example, comparing expression of gene 1 before and after treatment (not comparing gene 1 with gene...
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I am going to run qPCR for several target genes so that for absolute and relative quantification I need to make a standard curve. Do I need to use my target genes PCR product dilutions in order to...
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