Bahram Barati

10 Questions 22 Answers 0 Followers

Questions related from Bahram Barati

Is the decrease of ROS level in stress condition possible?

I have exposed microalgae cells to temperature stress and measure the ROS level using DCFH, but I found that the DCFH was significantly lower in stress-treated samples compared to control. How is...

31 March 2021 3,524 4 View

Is RNAi technology applicable for coronavirus?

One of the useful applications of RNAi technology has been discussed as a therapy. Based on the current state of this technology is this therapy methd currently used for humans? Is it more...

13 March 2020 1,446 17 View

Is CRISPR technology applicable in microalgae?

There are many ideas to create genetically modified microalgae for higher productivity, Why are the reports on this so little? I think now it is more possible with CRISPR technology. Any idea of...

28 May 2019 6,147 3 View

What is the most practical application of algae?

algae have been used in wide range of industries from being as food nutrient to bio-plastic. What is the most practical uses (commercially) of algae?

08 July 2018 1,788 3 View

Is it possible in Chlorella to have higher ROS level before heat stress?

I have measured ROS levels of Chlorella using DCFH before and after exposed to heat stress with following intervals 3h, 6h, 9h, 24h and 48h. The level of ROS before the treatment was higher. How...

10 December 2017 9,498 4 View

What are the advantages of understanding algae responses to stress?

Understanding algae responses to stress can help to improve our knowledge about algae adaptation strategies which can be used to engineer tolerant strains. Also, it can be used to...

10 April 2017 2,203 5 View

Which way is better to compare the gene expression level?

I am going to study the expression level of several target genes using qPCR. In my experiment, I expose microalgae culture to heat stress. then after I harvest the culture in different time points...

06 April 2017 8,592 6 View

What are the main predators of Chlorella?

Except rotifer

15 March 2017 8,552 2 View

To compare gene expression between treated sample and control using qPCR do I need to obtain primer efficiency of my target genes?

I am going to study the expression of 8 target genes in respond to heat stress using qPCR. For example, comparing expression of gene 1 before and after treatment (not comparing gene 1 with gene...

01 March 2017 3,587 4 View

What DNA to use for making qPCR standard curve?

I am going to run qPCR for several target genes so that for absolute and relative quantification I need to make a standard curve. Do I need to use my target genes PCR product dilutions in order to...

27 March 2016 8,398 8 View