Hi all,
I want to attach live e.coli (MG1655) on microbeads by charge. I found two options:
Both are positively charged and in the size I want. But one is hydrophilic and other is hydrophobic. Does hydrophobicity matter on e.coli attachment? My guess was that cell surface of the e.coli is hydrophilic but I found few papers attaching e.coli on hydrophobic surfaces so I started to wander.
Also, if I decided later to put lipopolysaccharides on the surface on beads, which of two beads would be easier? What would be the protocol?
Thank you for your time!
It depends. Liquid media grown bacteria usually are hidrophilic. When they are hidrophobic, i.e. micobacteria, they make agreggates at higher concentrations (OD more than 0.2). But you can essay this following
M G Hartley, M J Hudson, E T Swarbrick, A E Gent, M D Hellier, and R H GraceAdhesive and hydrophobic properties of Escherichia coli from the rectal mucosa of patients with ulcerative colitis. Gut. 1993 Jan; 34(1): 63–67.
or other papers
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