Seung Lee

6 Questions 3 Answers 0 Followers

Questions related from Seung Lee

How could I crosslink D-Mannose on polystyrene particles?

I am looking for D-Mannose coated microparticles in size roughly around 3-5um so I can attach e.coli cells. One I found was 50-150um D-Mannose Agarose beads but they are too big for my purpose. If...

15 January 2016 2,847 2 View

Is the cell surface of E.coli mostly hydrophilic or hydrophobic?

Hi all, I want to attach live e.coli (MG1655) on microbeads by charge. I found two options: Aliphatic Amine Latex Beads (Thermo, A37370) Amidine Latex Beads (Thermo, A37328) Both are positively...

07 December 2015 5,285 4 View

How can I attach E.coli on microhydrogel structure?

Hello everyone, I have this idea but not so sure about its technical procedure, so I am grateful if you could give me some advice/guidance. My goal is to make microhydrogel (or other...

23 October 2015 5,933 1 View

Could you give me your guidance of using Geneart for newbie?

Hi everyone, I need some help here and there ordering synthesized DNA from Geneart. I have basic understanding of synthetic biology but have no experience ordering synthesize DNA so I have several...

07 October 2015 8,411 1 View

Any advice in controlling protein secretion out from E.coli?

Hi everyone, I got this idea but don't know where to begin so my question will be broad. Is there any (naturally or engineered) mechanism of e.coli where it secretes (exocytosis) a specific...

12 August 2015 2,541 6 View

What are insoluble (precipitate) product that changes (or produce) color due to enzymatic activity?

Basically, I'm looking for something similar to LacZ/X-gal type of enzyme/substrate activity that produce insoluble (precipitate) product with color change but not with β-galactosidase. I want to...

19 June 2015 6,753 5 View