Factors that modulate the expression of Iba-1 in microglia?

Several papers talk about states (stressors) and pathological damage altering microglial behaviour and morphology and, as such, Iba-1 expression. However I'm struggling to find in the literature...

05 June 2018 5,929 3 View

Microglial morphology during apoptosis - beyond the cell body?

Current literature states that during apoptosis of microglial cells, the cell body can decrease in size, membrane blebbing can occur, and measures such as nuclear condensation, fragmentation can...

10 November 2017 6,136 2 View

Speed of chemotaxis - microglia in the CNS?

There is literature about the velocity of microglial processes movement in response to injury within the CNS, however I am looking for information on the timeframe/velocity of whole microglial...

10 November 2017 8,067 2 View

Will Annexin V tag apoptotic microglia?

I am trying to determine whether microglia are undergoing apoptosis in an experiment. I tried Active Caspase 3 was unable to get the antibody to work (IF).I was considering a different apoptotic...

07 August 2017 5,161 5 View

How to strip fluorescent antibody staining from tissue?

09 October 2016 3,036 4 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

05 June 2016 3,326 1 View

Optimal number of rodents required for in vivo wireless electrode study?

I am starting a new protocol surgically implanting wireless electrodes into the amygdala and hippocampus of C57 mice for a stress experiment and am wondering what the optimal number of animals...

01 February 2016 4,536 3 View

Can I record single unit activity and also determine a local field potential using one electrode in one experiment?

My PhD supervisor wants to look at both single unit activity of neurons within the lateral amygdala of C57 mice, and also wants to look at the local field potentials to determine theta rhythm...

01 February 2016 751 9 View

Why is my IHC microglia stained tissue getting what appears to be oxygen damage after mounting with Entellan?

03 April 2015 1,154 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View