Kristie L Smith

10 Questions 4 Answers 0 Followers

Questions related from Kristie L Smith

Factors that modulate the expression of Iba-1 in microglia?

Several papers talk about states (stressors) and pathological damage altering microglial behaviour and morphology and, as such, Iba-1 expression. However I'm struggling to find in the literature...

06 June 2018 5,998 3 View

Speed of chemotaxis - microglia in the CNS?

There is literature about the velocity of microglial processes movement in response to injury within the CNS, however I am looking for information on the timeframe/velocity of whole microglial...

11 November 2017 8,135 2 View

Microglial morphology during apoptosis - beyond the cell body?

Current literature states that during apoptosis of microglial cells, the cell body can decrease in size, membrane blebbing can occur, and measures such as nuclear condensation, fragmentation can...

11 November 2017 6,201 2 View

Will Annexin V tag apoptotic microglia?

I am trying to determine whether microglia are undergoing apoptosis in an experiment. I tried Active Caspase 3 was unable to get the antibody to work (IF).I was considering a different apoptotic...

08 August 2017 5,226 5 View

How to strip fluorescent antibody staining from tissue?

Does anyone have a working protocol or an idea of products I could use in the lab to strip fluorescent antibodies from fixed mouse brain tissue? I want to restain the same tissue with a Nissl stain.

10 October 2016 3,096 4 View

Is synaptophysin a presynaptic marker only?

I am just wondering whether a synaptophysin antibody will only stain presynaptic spines? I have access to both synaptophysin and PSD95 and am wondering whether both are necessary to look at pre...

07 July 2016 6,766 3 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

06 June 2016 3,382 1 View

Optimal number of rodents required for in vivo wireless electrode study?

I am starting a new protocol surgically implanting wireless electrodes into the amygdala and hippocampus of C57 mice for a stress experiment and am wondering what the optimal number of animals...

02 February 2016 4,597 3 View

Can I record single unit activity and also determine a local field potential using one electrode in one experiment?

My PhD supervisor wants to look at both single unit activity of neurons within the lateral amygdala of C57 mice, and also wants to look at the local field potentials to determine theta rhythm...

02 February 2016 815 9 View

Why is my IHC microglia stained tissue getting what appears to be oxygen damage after mounting with Entellan?

We have been using an IHC microglia stain on some mouse brain tissue after some experiments we ran.We followed our labs proven protocol for staining, and we dehydrate in graded ethanol and finally...

04 April 2015 1,214 4 View