Microglial morphology during apoptosis - beyond the cell body?

Current literature states that during apoptosis of microglial cells, the cell body can decrease in size, membrane blebbing can occur, and measures such as nuclear condensation, fragmentation can...

10 November 2017 6,136 2 View

Speed of chemotaxis - microglia in the CNS?

There is literature about the velocity of microglial processes movement in response to injury within the CNS, however I am looking for information on the timeframe/velocity of whole microglial...

10 November 2017 8,067 2 View

Will Annexin V tag apoptotic microglia?

I am trying to determine whether microglia are undergoing apoptosis in an experiment. I tried Active Caspase 3 was unable to get the antibody to work (IF).I was considering a different apoptotic...

07 August 2017 5,161 5 View

How to strip fluorescent antibody staining from tissue?

09 October 2016 3,036 4 View

Is synaptophysin a presynaptic marker only?

I am just wondering whether a synaptophysin antibody will only stain presynaptic spines? I have access to both synaptophysin and PSD95 and am wondering whether both are necessary to look at pre...

06 July 2016 6,707 3 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

05 June 2016 3,326 1 View

Optimal number of rodents required for in vivo wireless electrode study?

I am starting a new protocol surgically implanting wireless electrodes into the amygdala and hippocampus of C57 mice for a stress experiment and am wondering what the optimal number of animals...

01 February 2016 4,536 3 View

Can I record single unit activity and also determine a local field potential using one electrode in one experiment?

My PhD supervisor wants to look at both single unit activity of neurons within the lateral amygdala of C57 mice, and also wants to look at the local field potentials to determine theta rhythm...

01 February 2016 751 9 View

Why is my IHC microglia stained tissue getting what appears to be oxygen damage after mounting with Entellan?

03 April 2015 1,154 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View