I need to prepare or dilute TGF-B3 in the mentioned solutions: sterile-filtered 10mM acetic acid with 20% ethanol, or 4mM HCl.
I have just searched through videos from YouTube, sterile filtration is to simply filter any solutions through a 0.2um pore size filter unit using a sterile syringe, to remove microorganisms. I am wondering if this so-called "sterile-filtered" solution safe for use to dilute or reconstitute the growth factors and used for cell culture? Are they considered aseptically clean for cell culture work? I hope I will not introduce any contaminants to my neuron differentiation work which is very tedious. Thank you.
Hello
Using 0.2 micron filter unit you are assured that the filtrate is free of all microorganisms. The use of 0.2 um filter will exclude E. coli, and other bacteria, cysts, parasites, rota virus, norovirus, and other viruses, ultrafine particles, sediments and other biological hazards from the filtrate.
So you can go ahead and sterile filter your solution using 0.2um syringe filter. It is very much Safe for cell culture.
The filter comes in various forms like Polyethersulfone (PES), Polypropylene (PP) and Polytetrafluoroethylene (PTFE). Make sure you use the right filter for your application since you are using an acid as a diluting or reconstituting solution.
I hope this is helpful.
Best Wishes.
Malcolm Nobre Thank you very much for the information. To be safer, can I keep the sterile-filtered solution under UV light in the biosafety cabinet? Thank you.
Hi Teo Ting Ting
There is no need at all. I use sterile-filtered solution immediately without any problem. I never get contaminations in my cultures. It seems you are still not confident using sterile filtered solutions directly. But for your satisfaction you can keep sterile-filtered 10mM acetic acid with 20% ethanol, or 4mM HCl under UV in the biosafety cabinet for sometime. There should be no problem.
All the Best.
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