When collecting data to conduct CFA, can I collect an expanded set of items and then limit the CFA to the reduced set of items from the EFA results?

I have been working of developing a new measurement tool. I have followed all the standard scale development steps / best practices and have a reduced set of items (from 31 down to 22 items via...

02 September 2019 2,598 3 View

Why am I getting increased transformation efficiency in yeast transformation protocol when I have changed nothing ?

I have been working is S.cerevisiae for about 2 years. Up until about 8 months ago, I would have successful plasmid transformations with about 30-100 colonies per plate depending on the size of...

28 January 2018 1,321 3 View

Tau Protein Western Blot Analysis in ImageJ

Can anyone explain how to analyze western blots that contain multiple bands for a target protein in ImageJ? This image is for Tau 5 (total tau) but this also happens for pSer396. This is...

01 July 2017 8,874 6 View

How much folate is provided to a plant from a microbe symbiont?

I work with methylotrophic bacterial plant symbionts. I know that the bacterial symbionts produce and release folate into the soil that the plant will take up. Does anyone know about how much of...

30 August 2016 9,660 1 View

Live decapitation preferable to isoflurane or CO2 asphyxiation?

I was speaking with our University's vet and she mentioned that live decap is the better route to take if you're planning on doing any histology after death. Does anyone have any further...

19 July 2016 4,669 6 View

Does anyone know whether hTERT-RPE1 cells express bestrophin-1 protein? Do you know whether they polarise or differentiate?

We are trying to find an RPE cell line that expresses bestrophin-1 protein. We have established that hTERT-RPE1 and ARPE-19 express the RNA however do not appear to produce the protein. We would...

06 October 2015 712 2 View

Where does my AU Rich Element (ARE) region begin?

Hi, I'm working on a suspected ARE region in a 3'UTR of a transcription factor. Using the ARED website we've found that there are two "AUUUA" pentamers in our 3'UTR that are close together that...

15 February 2015 9,005 4 View

Is anyone familiar with nested PCR?

I started running nested pcr this week using Extract N Amp. This is my first time doing this, and after the second reaction, my water negative showed positive bands. I will look into contamination...

25 September 2014 7,779 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Can I publish my published dataset (data-report article) later as an original article?

I want to quickly publish a data-report .Because there is a lot of data and it will take time to create the original article. But my main goal is to use that data-set in the original article. Is...

02 February 2021 9,779 7 View

Can someone help me a with a rich dataset for early prediction of Dementia?

Hi, I am interested to carry out a thesis on early prediction of Dementia using csv/excel type data. Can someone please assist?

26 January 2021 7,381 3 View

Does anybody has the biodot zx1010 manual?

25 January 2021 6,257 1 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View

The > 91 Years long collective IQ Blunder ?

Never before was there a bigger blemish. Namely: Not to collectively underastand Zwicky's discovery of Cryodynamics, as his sister to deterministic Thermodynamics came to be called later. Dec....

30 December 2020 8,300 5 View

Is explanatory sequential design the right design for my research?

I am interested in finding the effects of technology on the learning achievement of students. The data collection will include pre and post test scores of the experiment group and later...

26 December 2020 3,757 4 View

How do I use clinical BioBERT for relation extraction from clinical text?

Hola I'm working on a project that deals with clinical named entity recognition, relation extraction etc. I'm currently using Scispacy library for NER work. However, I'm searching for a open...

16 December 2020 5,701 1 View