I tried normal and reverse phase HPLC methods to achieve separation of methylphenidate glycerol esters(positional isomers) but with no luck.Does anyone have any methods or ideas which i can use.
Dear Sir. Concerning your issue about the separation of methylphenidate glycerol ester isomers using HPLC. Methylphenidate (MPD) is a chiral drug with stimulant action, used for the treatment of attention deficit hyperactivity disorder (ADHD) and narcolepsy. It has been used improperly and illegally, as it is assumed to improve cognitive performance. It was established a method of determining methylphenidate isomers in human plasma by separating racemic methylphenidates from the mixture using high performance liquid chromatography (HPLC) with a diode/UV detector. The separation was performed on a Chirobiotic V2 column, a mobile phase of methanol/ammonium acetate (92:8, V/V; 20 mM pH 4.1) and flow rate of 1 mL min-1 ; retention times were 7.0 and 8.1 min for l-MPD and d-MPD, respectively, detected at 215 nm. The HPLC method was validated via linearity, precision, accuracy and recovery. The method was suitable for separation and determination of enantiomers of methylphenidate in human plasma and is considered precise and accurate. I think the following below links may help you in your analysis:
http://www.sphinxsai.com/2017/ph_vol10_no2/1/(114-119)V10N2PT.pdf
http://rushim.ru/books/lekarstva/art-of-drugs-synthesis.pdf
Thanks
Hello Isam Eldin Hussein Elgailani,
Thank you for your answer. I am going to try that method and see if it works.
For more information I am attaching the mechanism and structures of Methylphenidate glycerine esters taken from a publication
- Badges
- Science method