Chantal Kuhn

4 Questions 26 Answers 0 Followers

Questions related from Chantal Kuhn

How would you inhibit proinflammatory cytokines in mice for a couple of days only (preferentially orally)?

I was wondering what would be the best way to inhibit local production of pro-inflammatory cytokines (IL-6, IL-17, IL-1, IL-15 ...) in the gut of mice for a couple of days (5-7days) without...

04 April 2013 3,330 3 View

Which collagenase to use for isolation of DCs from the intestinal lamina propria of mice?

Normally I isolate DCs from lymph nodes using Collagenase D and DNase I. However I know I plan to try isolation of DCs from the lamina propria and some protocols suggest Collagenase VIII instead....

03 March 2013 669 5 View

How would you increase the survival of freshly isolated mouse DCs (pDCs on one hand and cDCs on the other)?

I would like to study the influence of different dendritic cells on the polarization of T cells in vitro. However I have a very poor survival of DCs (especially pDCs) when I culture them in vitro...

03 March 2013 7,833 3 View

Is there a way to detect and sort antigen presenting cells that present an autoantigen via MHC class II?

I would like to sort antigen presenting cells that present the autoantigen MOG35-55 restricted by MHC class II (I-Ab). Does someone know if there are antibodies / tetramers that stain the complex...

03 March 2013 682 6 View