Is peripheral blood mononuclear cell (PBMC) storage possible for flow cytometry for 2 months or more?
For example, we collect the blood sample and isolate PBMC, and then store it for the next analysis (i.e., flow cytometry) for several months, then analyze it. Is it possible? Will the quality decreases?
Please ask..
Hi Raoof,
If I understand you correctly, you would like to know if it is possible to store freshly isolated PBMCs for flow analyses at a later time.
It is possible. You just have to be careful with your freezing/thawing protocols. Freeze the cells in an appropriate medium (FBS-10% DMSO or Cyrostor) and thaw them carefully using established protocols. If you are just looking at the expression of surface markers by Flow, freezing/thawing would not have a big impact. If you are looking at the expression of cytokines, the signal does go down with freezing. It is always better to work with freshly isolated PBMCs, in this case (but not always logistically feasible).
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