Many intracellular processes, such as DNA replication, repair, cell cycle transitions, nucleus and spindle assembly etc., can be recapitulated and studied in a cell-free setting, e.g. in Xenopus egg extracts. Does anyone know whether oxygen concentration or light spectrum have an effect on any of the above or other processes modeled in cell-free systems?
Thank you.
In principle, oxygen concentration or light spectrum is a important factor of DNA repair, replication, as well as recombination in living system. Thus, the question invokes whether molecular physiology is relevant for a model system in terms of variations measurement. Considering a cell-fee system is a model of life soup per se, the robustness of interested measurement is largely context-dependent. It appears to be loss of compartmentalization for cell free system thereby such a distorted organization leading to a distinct chaotic signaling system which is unknown. Thus, an intricate system is required to model oxygen concentration or light spectrum as a factor especially considering the reaction speed and the time to reach equilibrium. Together, it might be crucial to employ an oxygen concentration or light spectrum sensitive enzyme to examine all these speculations. I was wondering which protein is ideally suitable.
- Badges
- Science method
- Similar topics
- Mathematics
- Statistics
- Statistical Techniques
- Structural Equation Modeling