Is there any good coupling interfaces available for ANSYS (FEM) and EDEM (DEM)?

Actually I am thinking to apply it for a bulk of non-spherical particles in a storage faciltity

21 May 2018 8,719 4 View

True Nrf2 band in western blot ?

I am using a Nrf2 Antibody (A-10): sc-365949 from santacruz which is a new replaced monoclonal antibody. With this antibody, I am getting an intense single band in my control sample at around...

10 April 2018 8,925 3 View

GROMACS and flat-bottom position restraints

Hi, I want to know whether the flat-bottom position restraints can be applied only for the pull code in umbrella sampling or it can be use for NPT or MD simulation of GROMACS (not umbrella...

23 May 2017 8,190 1 View

Does anyone have and can share a sample questionnaire for an INTERPRETIVE STRUCTURAL MODELING technique?

I need to make an analysis of the barriers to sustainability of supply chain practices using ISM. I don't exactly know the format of such a questionnaire design. If anyone knows or has a sample....

19 December 2016 1,647 2 View

Oscillator function model for eosin yellow?

19 October 2016 8,647 9 View

Can we know the doped materials surroundings voids by refinemnet the XRD using MAUD?

From the refinement by MAUd we can generate CIF file which help to get the crystallographic structure of the material. But in case of doped material how the refinement should be changed to...

22 July 2016 4,191 3 View

How can I convert distribution of interface state density in terms of voltage to energy distribution?

I use hf C-V and conductance method to determine the interface state density of MOS structures in terms of voltage. I am not able to show the distribution in terms of energy. How do i convert the...

02 April 2015 4,463 3 View

What are the markers for iDC derived from human PBMC stimulated with IL-4 and GM-CSF?

I have isolated PBMC from human blood and allowed to adhere to plastic surface for 3 hours and then washed with media to get rid floating cells.Then adherent cells were stimulated with IL-4 and...

20 January 2015 3,341 13 View

How long does it take to degrade target transcripts by siRNA?

Typically, the effect of siRNAs are assayed after 48hrs. During this period, the siRNA is expected to "keep degrading" the existing as well as newly synthesized transcripts. Now, if there is a...

16 December 2014 5,021 4 View

Do eRNAs remain attached with the template DNA or are detached and float around?

The last couple years have seen several reports of enhancer RNAs (eRNAs and similar transcripts) that aid enhancer- and activator-dependent transcription. Arguably, a chunk of 5' end of the eRNA...

26 August 2014 2,518 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View