Is osmolality and pH important in subcutaneous injection solution?

I making a subcutaneous formulation of conc. 20mg/ml in aqueous solution with cosolvent NMP. The pH of formulation is 6.55 and osmolality is 1352 mOsm that is the formulation is hypertonic. Is it...

09 June 2019 9,509 1 View

Is there any good coupling interfaces available for ANSYS (FEM) and EDEM (DEM)?

Actually I am thinking to apply it for a bulk of non-spherical particles in a storage faciltity

21 May 2018 8,719 4 View

True Nrf2 band in western blot ?

I am using a Nrf2 Antibody (A-10): sc-365949 from santacruz which is a new replaced monoclonal antibody. With this antibody, I am getting an intense single band in my control sample at around...

10 April 2018 8,925 3 View

GROMACS and flat-bottom position restraints

Hi, I want to know whether the flat-bottom position restraints can be applied only for the pull code in umbrella sampling or it can be use for NPT or MD simulation of GROMACS (not umbrella...

23 May 2017 8,190 1 View

Does anyone have and can share a sample questionnaire for an INTERPRETIVE STRUCTURAL MODELING technique?

I need to make an analysis of the barriers to sustainability of supply chain practices using ISM. I don't exactly know the format of such a questionnaire design. If anyone knows or has a sample....

19 December 2016 1,647 2 View

Oscillator function model for eosin yellow?

19 October 2016 8,647 9 View

Can we know the doped materials surroundings voids by refinemnet the XRD using MAUD?

From the refinement by MAUd we can generate CIF file which help to get the crystallographic structure of the material. But in case of doped material how the refinement should be changed to...

22 July 2016 4,191 3 View

How can I convert distribution of interface state density in terms of voltage to energy distribution?

I use hf C-V and conductance method to determine the interface state density of MOS structures in terms of voltage. I am not able to show the distribution in terms of energy. How do i convert the...

02 April 2015 4,463 3 View

What are the markers for iDC derived from human PBMC stimulated with IL-4 and GM-CSF?

I have isolated PBMC from human blood and allowed to adhere to plastic surface for 3 hours and then washed with media to get rid floating cells.Then adherent cells were stimulated with IL-4 and...

20 January 2015 3,341 13 View

How long does it take to degrade target transcripts by siRNA?

Typically, the effect of siRNAs are assayed after 48hrs. During this period, the siRNA is expected to "keep degrading" the existing as well as newly synthesized transcripts. Now, if there is a...

16 December 2014 5,021 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View