PhD Bio renewable energy research topic?

I am having challenge in picking a research topic that focuses on bio-renewable energy probably because a lot of feedstocks and methods have been research on. I would like suggestions on topic...

27 October 2020 9,898 2 View

How to Separate saturated FFA from Unsaturated FFA?

Starting from soybean oil, how can only unsaturated free fatty acid be separated from the saturated free fatty acid in the Oil? The purpose of this is to synthesis Alkyd resin with just only the...

11 October 2020 5,149 3 View

How to syntheses Alkyd resin with bright colour from dark brown rubber seed oil?

All of the alkyd sample from rubber seed oil I have synthesized is always black in colour, such colour is not good for the production of white oil paint. I need help on what to do to achieve...

10 October 2020 9,860 1 View

What would be an appropriate parent-report tool for measuring circumscribed interests in young children with autism?

I'm looking to get an idea of the intensity of circumscribed interests for young children (3-6 years) with autism. Does anyone know of an appropriate parent questionnaire that can be used to...

14 January 2019 7,013 3 View

In gas-liquid-liquid STR, how is turbulence modified and what is the impact on drag felt by the gas bubbles?

In the CFD prediction of gas hold up in gas-liquid STR, it is necessary to take into account the observation that turbulence increases the effective drag felt by gas bubbles thereby reducing their...

17 June 2018 3,014 2 View

Grid size vs bubble size in Euler-Euler simulation of gas-liquid flows in stirred tank reactors?

In the simulation of stirred tank reactors, it is necessary to finely resolve the region near the impeller so as to adequately capture turbulence properties such as the energy dissipation rate....

07 February 2018 9,687 2 View

What is the optimum time for transfection PEI system?

I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.

26 October 2017 1,650 2 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

20 May 2015 9,719 3 View

In ELISA, why does the IgG standard only bind when I coat plate with anti IgG,.,.but does not bind with my protein of interest.?

I have ran an elisa assay with one side coated with anti igg and one side coated with SEA protein,.,.i put standads in both sides,.but only the side coated with anti igg showed to have working...

16 December 2014 4,993 2 View

How can I prevent mold and yeast contamination of cell cultures?

26 November 2014 1,049 16 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View