Is miRNA detection by stem-loop PCR 100% believable?

More Ramamoorthy Sankaranarayanan's questions See All

Can we eradicate the plant viruses (Like small pox and polio virus eradication)? Is it possible?

For a year or so I am thinking about the eradication of plant virus. But I have many questions on it. Please give me your suggestions/views/opinions. Articles you come across if any regarding...

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Does plant positive sense ssRNA viruses code microRNA?

It is well known many viruses code miRNA for verity of purposes. However, in my opinion, miRNA of plant +ssRNA virus is sparse. Please share your views/articles to help me. Thanks in advance.

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Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How do I increase the yield of my PCR product?

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Research on Supply Chain Management in Indian context?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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How to address this issue in the analysis of Real-Time PCR results?

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Expression cloning by using cDNA,do I need to modify the cDNA first?

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Runs of nucleotides in Sanger sequencing?

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Can thermal cycler machines be problematic during PCR?

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Calculating volume of DNA required from DNA concentration?

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Hisham Abu-Ali

Dear Ramamoorthy Sankaranarayanan

The Stem-Loop RT-qPCR Not just PCR is an Efficient Detection Tool

Try this article link to get much more information

Article Quantitative stem-loop RT-PCR for detection of microRNAs

Alexander Sasha Vlassov

The stem loop technology works great for miRNA detection/quant. We used it quite a bit in the lab- with Applied Bio assays