Hi
I am doing a DIG labelled probe based southern blot analysis of rice transgenics and it is recommended to digest at least 10 ug genomic DNA in a 150 ul digestion reaction so as to dilute out any possible salt or contaminants during the digestion. The digestion appears to be good and complete but the next step is to reduce the 150 ul volume to 15-20 ul so that I can load it onto a gel.
The question is whether I can lyophilize ( freeze-dry) my restriction digest and then resuspend overnight in 15 ul TE without any shearing of my large dna fragments. I am doing an insertion copy number analysis so it is imperative that I don't have any partial digests or too much random shearing.