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Questions related from Nishat Passricha
Can anyone suggest me a tool to check the nature of promoter (Inducible or constitutive) through bioinformatic or computer tool.
01 January 2019 8,905 3 View
Dear All I have a set of primer in diluted form, . I want o know the sequence of that primer set. Which technique should be useful for identification of exact sequence.
04 April 2018 5,925 0 View
Dear all I have approx 1000 genes for which I want to design the cloning primer for pENTR™ vector (gateway Cloning). Can anybody help me to design these primers in bulk as matching the tm for...
02 February 2018 6,492 1 View
I am using baculovirus system for producing protein in SF21 cell. During this I found the exact band during western with anti HIS antibody in control cell line. Plz give your suggestion ...
11 November 2016 870 3 View
I am using a Baculovirus system in insect cell sf21 for the production of recombinant protein. I did the transfection approx 80 hour before now its showing the needle like structure in media, My...
07 July 2016 9,225 4 View
I am working on SF21 cell lines for a recombinant protein. During this I got several viral stock like P1, P2 and.....One most reliable method is by Plaque assay. But this is very long and time...
07 July 2016 9,277 2 View
I was making the solution of SS DNA. During this I have to sonicate to make working solution. This solution is not working for yeast two hybrid. I have changed all the yeast strain, DNA and medium...
01 January 2015 5,567 3 View
