Donald James

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Questions related from Donald James

Is lyophilization of a genomic DNA restriction digest a good way to concentrate the sample without inducing any shearing ?

Hi I am doing a DIG labelled probe based southern blot analysis of rice transgenics and it is recommended to digest at least 10 ug genomic DNA in a 150 ul digestion reaction so as to dilute out...

04 April 2016 5,039 3 View

How to make your SSH library more representative ( that is to get more clones of genes which are induced by drought stress in the SSH library ) ?????

I am doing SSH ( Suppression Subtractive Hybridization ) of drought stressed Pennisettum glaucum .I have obtained 12 contigs and around 34 singletons from some clones which i randomly picked...

12 December 2011 9,317 1 View