How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Direct viral RNA detection (RT_PCR)?

Is it possible, in your opinion, to detect RNA using RT-PCR but without RNA extraction? Considering for example a pharyngel swab sample, it is possbile to do a pre-treatment in order to preserve...

29 March 2020 7,576 5 View

Parameters selection for HDBSCAN algorithm in Supervised setting ?

I would like to know if there is any kind of research on the parameter selection (minpoints) for the HDBSCAN algorithm, in the context of having a training set with only normal samples and a test...

06 March 2020 3,487 3 View

How can I solve convergence problems of a dynamic analysis of an earth dam with opensees?

Hello everyone! I'm trying to model a earth dam with opensees. To do TCL file, I used GiD ProblemTypes for 2D Slope Analysis Input File Generation. When I do dynamic analysis with small...

03 September 2019 2,508 2 View

Are ACO and PSO ranking based feature selection approaches?

Hello, I've just came across these two algorithms and I was wondering whether there are any available versions for ACO and PSO as ranking-based feature selection approaches? Any comment would be...

18 July 2019 4,528 8 View

How can I calculate the aerodynamic characteristics of horizontal tail in FLUENT?

22 May 2019 6,252 3 View

How can I overcome the 230/260 low ratio problems after DNA clean up? and why do you think i got this problems?

Hi to everybody, I am doing the cloning experiment but I have problems before performing the digestion as my clean-up products have a 260/230 ratio of 0.5. There is someone available to explain...

03 May 2019 5,354 8 View

Where can I find the right CHARMM22 parameters for my paramagnetic complexes?

Hello, I'm trying to model some gadolinium complexes through molecular dynamics using the parametrized CHARMM22 force field as described in International Journal of Quantum Chemistry, Vol. 73,...

04 April 2019 6,889 1 View

How can Principal component analysis (or factor analysis) be applied on a questionnaire score, and how can the results be intepreted?

During my research, I asked several companies to complete a questionnaire to measure they risk maturity. At the end of the questionnaire, each respondent receives a score from 1 to 5 (1=low...

26 March 2017 491 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

I need help in wavenet matlab code, any one help me?

I tried to use wavenet (wavelet and ANN) INSTEAD OF ANN FOR THE PURPOSE OF CLASSIFICATION

06 December 2020 864 5 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

SIMULATION of TURBULENT PREMIXED FLAMES of CH4/NH3/air in Ansys Fluent?

Hello, I am doing SIMULATION of TURBULENT PREMIXED FLAMES of CH4/NH3/air in Ansys Fluent. I am using the following: Partially Premixed Combustion Model; Chemical Equilibrium; Extended Coherent...

17 November 2020 5,126 2 View

How top apply feature extraction for eeg ?

Dear community , I tried to extract features using continuos wavelets transform using python on my data , but I faced some problems ; my dataset are sleep recordings for 10 patients (physionet...

06 November 2020 895 12 View

How to select the frequency range in MATLAB Wavelet toolbox?

I have a continuous, time-domain noisy signal and want to decompose into low-frequency (LF) and high-frequency(HF) components using Wavelet Toolbox. But, I am unable to select the frequency range....

29 October 2020 5,219 5 View

Why 256x256 medical image gives me 256x256x3 in matlab?

I need a 256x256 pixel image to use it in my project. I downloaded images from the internet, but when I read the image, it stored in a 256x256x3 variable (color image format PET images). the NSCT...

11 October 2020 686 13 View