How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to do Statictical analysis for unequal sample sizes?

Hi Everyone, I have a dataset from proteomics analysis of treated cancer cell line, I have three replicates in treatment time-points and two replicate in control condition. Now, How can I...

01 January 2021 4,055 3 View

The reason for this error in Norsar software???

This error is in the interactive wavefront Tracer section. Tracing process failed: Received job failed indication from initial configuration job: Tracer process (C:/Program Files/NORSAR...

10 July 2020 6,344 3 View

How can I dry aluminum flake without the danger of ignition?

In order to produce Al flake in the ball mill, it is kept under an organic solvent. how can we remove it later from this solvent?

06 July 2020 6,232 7 View

Has anyone ever taken crystal of meta boric acid or B NMR ?

I'm making a boron-containing by-product which in only soluble in DMSO. I've been trying to make a crystal of that. I'm kind of sure is meta boric acid and I have a peak at around 20 ppm in B NMR.

05 February 2020 2,197 2 View

Which additive can create tone (especially green) in white ABS masterbatches to omit the undesirable yellow color ?

white ABS masterbatch usually has bluish or greenish color. optical agents lead to green tone in white masterbatch and omit inherent yellow color of ABS but will decompose during the process.

11 October 2019 3,740 1 View

How to choose the proper neural network Classifier in MATLAB?

I want to use a neural network classifier to separate patients and healthy persons by some parameters. and then test validation of the classifier. Is svm a proper tool? And how can I test the...

21 October 2018 8,968 8 View

Dose anyone know why ht29/5 fu resistant cells don't grow?

17 November 2016 6,149 3 View

How can I solve this RACE PCR problem to make the band visible?

01 January 2015 8,545 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View