Is it possible to use RNase to inhibit micro_RNA INSIDE Evs? Or does it inhibit miRNAs secreted from EVs. This is crucial for my research.Thanks

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Fatemeh Momen-Heravi

Yes it is possible. You can introduce miRNA inhibitor to the EVs by using electroporation or transfection reagents directly on the isolated EVs. You can use the dual luciferase assay to confirm the inhibition and optimize the concentration of the miRNA inhibitor.

Eman Thabet

thank you... i was wondering if there was a way that doesnot require transfection.