Dear colleagues,
I have a small question about competent cells protocol
Could I use the protocol to make competent cells
E. coli for S. aureus ?
If you have further suggestions or protocols
(the one that you already did well)
please share with me
Thank you so much ,
Hopefully someone can give you more specifics related to S. aureus but -
Probably not. Gram positive bacteria typically require special treatment to weaken their cell walls before they'll take up plasmids by electroporation. They also usually require a media with high osmolarity (often achieved with something non-ionic like a sugar) to protect them from rupture post-electroporation.
Dear Alexandra Johnson ,
Thank you so much for your good comment. After reading your comment, I have found some references on agreement with your suggestions. Thanks a lot ,
- Badges
- Science topic