20 Questions 15 Answers 0 Followers
Questions related from An Nguyễn Hoàng
Dear colleagues, Long time no see ... Today I have a question regarding methods to illustrate the bioavailability of peptide-mimic agents in vivo by using mammalian cells instead of other...
28 March 2025 7,321 4 View
Dear colleagues, I am trying to figure out the tight junction of epithelial cells induced by a drug . Whether must I use a specific staining or conventional cell membrane one sufficiently...
27 March 2024 1,025 0 View
hi dear i have an essential question regarding bandscore to evaluate nephrotoxicity of a specific chemical structure there are some in references but not the only one , i wonder could we choose...
14 September 2023 1,461 0 View
Dear colleagues, I am on the way to construct LPS structures of some bacterial strains Do we have any software to remodel online up to chemically interactive bond (including adding remedy...
14 July 2023 4,257 0 View
Dear colleagues, Currently I have difficulty to grow S. pneumoniae. Somehow it does not grow well. The OD600nm gets only upto 0.5 - 0.8 after 5 days of innoculation with TSB + 5% CO2 On testing...
28 June 2023 7,537 3 View
Dear colleagues, How can you define the intrinsic resistance of bacteria against anti-microbial agents ? "Intrinsic resistance is when a bacterial species is naturally resistant to a certain...
01 May 2023 2,549 4 View
dear colleagues , this question could be not simple , I want to ask that which are the mechanisms could explain for the swollen macrophage after being infected ? the negative control showed...
07 September 2022 973 0 View
dear colleagues , i have got a quite simple question on today , which is the mechanism of S. aureus USA300 resistance against kanamycin at very high concentration 64 ug/mL ? could it be...
03 September 2022 8,004 0 View
dear colleagues , i have a question about dynamic light scattering sample which is the most suitable dilution ratio is it based on the diameter of target nano-scale structure of each sample ?...
07 August 2022 9,849 22 View
hello dear colleagues, i would like to have one small question : after being competent for example by calcium chloride protocol could it be any change in prokaryotic chromosomal DNA ?...
12 July 2022 9,671 0 View
hello dear colleagues, i am finding the protocol to measure the intracellular calcium concentration of mammalian cells if you have any suggestion please let me know those protocols already...
21 June 2022 6,672 0 View
hi colleagues , i would like to ask a question about : methods to deliver CRISPR/Cas9 into cells those current update of protocols have reached a huge diversity however, which could be the...
18 June 2022 902 0 View
hello dear colleagues who have the same view with my question, i would like to ask could it be mutation(s) in 5' / 3' UTR being triggered by drug / chemical exposure ? if i only checked...
29 April 2022 3,405 0 View
dear all who share the same view with my concern , i am finding the softwares which can predict the potential on/off-target cleavage sites of multi-guide sgRNA (prokaryotes) (cas9) my purpose is...
23 April 2022 8,539 2 View
hello dear colleagues, this question I would like to ask about apoptosis / necrosis phenomenon regarding of well-known biomarkers , in case we have not checked biomarkers yet , for instance ,...
21 April 2022 2,062 3 View
dear all who have the same view with my concern , i would ask a small question that how can we enhance the proliferation of quiescent zone of spheroid ? thank you for your suggestion ,
08 April 2022 816 2 View
hi all , whom be interested with my question do you have any good suggestion of protocol to transfer dose equivalently from in vitro to in vivo (mice) ? many thanks and most sincerely
03 February 2022 9,164 0 View
hi all , whom have been on the same view as my questions , 1. could you help to suggest for me some methods to improve the proliferation of keratinocytes (HaCaT cells). my problem now is that...
27 February 2021 6,791 6 View
Dear colleagues, I have a small question about competent cells protocol Could I use the protocol to make competent cells E. coli for S. aureus ? If you have further suggestions or protocols...
01 January 1970 7,813 2 View
Dear colleagues, Hopefully, everything is going well with you. I am so thankful if possible you could share with me your experimental experiences / case reports regarding drug resistance due to...
01 January 1970 8,531 0 View
