I want to do benzidine staining in order to distinguish erythrocyte with other cell types. Thanks.
I did an experiment for a positive control phos-tag system to detect phosphorylated AMPK in min6 cells. I incubated min6 cells for 12h with metformin to induce AMPK phosphorylation then check the...
06 January 2024 7,752 1 View
can a gene cause slow growth phenotype in vitro but also in qPCR has down regulation on p16?
05 November 2023 1,121 0 View
I want to identify a secretory protein that is secreted from min6 cell in the supernatant can anyone provide protocols? Firs I want to get conditioned media and then precipitate after that I will...
05 October 2023 9,963 1 View
does serum starvation need to be done in the exponential growth cells? or is it okay to do it 20hr after seeding? my cells is a slow growth type cells that has ~48 hours doubling time
20 July 2023 2,308 0 View
I did cell synchronization by serum starvation to my cultured mammalian cells for 24 hours and I did FACS analysis, however I found no G1 arrest, I prolong the starvation for 72 hours but I got...
07 July 2023 2,915 1 View
Is it possible that a KD cells showed reduced cell proliferation by WST but during FACS analysis it did not show any change of cell cycle? apoptosis checking by cleaved caspase 3 western blot also...
26 June 2023 3,670 1 View
I am a newbie in FACS analysis: so I used this reagent from dojindo called cell cycle blue reagent and do staining in live cells (not like PI) they stated that fixation is not needeed, the problem...
08 June 2023 6,644 6 View
I am now studying a protein, this protein might affects cell proliferation because I performed a Knock down experiment and I found that the cells proliferation rate is reduced when this protein is...
08 June 2023 1,586 2 View
By retroviral over-expression system I couldn't get the same amount of over-expression efficiency (increased expression in protein level as detected by western blot). I tried overexpressing a...
28 May 2023 7,350 3 View
I performed PCR for genotyping but I could not amplify one of the sample while other samples is amplifying just fine... even I put + and - control, the + ctrl is amplifying well... what could be...
14 May 2023 3,111 8 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View