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Questions related from Kent Fung
Hi all, I have a ex vivo drug screening system for leukaemia, but I realised some samples contains both leukaemia and a significant amount of healthy cells (5-30%). I am looking for a no-wash (or...
05 June 2024 3,753 0 View
Hi all, I am currently helping my colleague to do a drug screening assay. We have use dTag to "turn off" the protein expression of our protein of interest and treat the cell line with drugs...
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Hi all, I have been doing drug screening on clinical samples and multiple 384-well plates are used for each samples. However, when we look at the cell number in the DMSO control on each plate,...
06 December 2023 4,457 7 View
Hi Community, I am planning to express 6 human proteins in a mouse cell line. They are from 400 to 800 bp long. I want to use three vectors, each containing two CDS connecting with 2A peptides,...
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Hi Fellow Researcher, I understand the title isn't clear enough. Allow me to explain. I have two pairs of cell lines (pair 1: cell line A, cell line A with WT gene 1; pair 2: cell line A, cell...
12 January 2023 7,197 1 View
Hi fellow researchers, I have been using this MSC cell line (hTERT immortalised) for some time. However, they have very different growth pattern (See attached) and I have done test on what...
22 November 2022 190 4 View
Hi everyone, I have a blood sample that was RBC-lysed with ammonium chloride solution and frozen down in FBS with 10% DMSO in multiple vials. For the reason of the atypical composition of the WBC...
18 December 2018 691 0 View
