Hello,
My project is trying to examine the effect of a bioactive component on diabetic-induced brain damage. we have high-fat high sugar induced diabetes mice model, then we do some analysis of mice brain. At the mean time, I want to mimic a same condition in cell model, to do other analysis. The cell lines could be SHSY5Y or PC12 cell line. Does anyone has any idea?
Thank you in advance for your help!
I don‘t think this will make sense. Cell lines are so different with bains.
This paper describes use of high glucose and high fatty acid DMEM medium. You could also add LDL (low density lipoprotein). Article Lipid Mixtures Containing a Very High Proportion of Saturate...
Dear Rosa Li,
yes, it is possible. we did an experiment on the PC12 cell line. first, we applied an MTT test to find out the appropriate concentration of Glucose for the experiment. our results showed that 300 mM of Glucose killed the cells. you should find the concentration and then for examining the effect of a bioactive component, you should add the appropriate dose of the component and after 5-6 hours you should add the suitable concentration of Glucose to your 96-well plate.
I want to construct a high fat cell model too. And I have tried to use lipid mixture (obtained from SIGMA) to treat esophageal cancer cells, this treatment did not facilitate cell proliferation significantly but can slightly increase lipid content in cells, since Oleic acid was thought to increase lipid droplets. I am not sure whether this protocol is suitable and reliable.