I would expect sequencing to work as all the sequence labelled fragments will be shorter than the full length labelled amplimer when you are sequencing with a non labelled reverse primer. Using the labelled primer as a sequencing primer will be a problem as the mobilities of all of the fragments from the sequencing reaction will be wrong due to the charge on the FAM label.

I have use exo sap and other methods and there are a few situations where exo sap does not work while gel purification always works but I would always use exo sap as it is quick, easy and almost always works

Dear Paul,

thanks a lot for this really helpful answer!

Gertrud

always I agree with @Paul Rutland

regards

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regards

should be possible, but what about getting rid of the label? cut the label of - as long as it swims around in the sequencing "soup" it will do no harm, so you will not lose material by a purification step...