Is it possible to separate diffierent subregions of anterior olfactory nucleus(AONm,AONl,AONd,AONv,AONe) by immunohistochemistry?

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Wasiu Gbolahan Balogun

I would suggest you use landmark, you can use the stereotaxic coordinate, finding it difficult to upload here by you can download the rat brain in stereotaxis coordinate by paxinos and WATSON

Yadong Gang

According to what I have learnt, we can hardly separate diffierent subregions of anterior olfactory using immunohistochemistry technique. Have you ever tried to use NeuN antibody?