Depends which amino acid you are speaking of; pTyr, pSer, pThr, pHis, pLys, pArg etc...
Most likely one of the phosphoesters? pH extremes will work differentially depending on which amino acid you care about. Why not use a phosphatase (e.g. alkaline phosphatase etc…)?
Thank you for your answer Mr. Fuhs. I currently do not know which Amino acid sequence is phosphorylated. I plan to investigate interactions between two proteins and most likely they do not bind when one of them is phosphorylated. Since I also want to see whether it could be used as a therapy against a disease, I think using phosphatases and kinase inhibitors are detrimental. That was why I am trying to figure out if there are milder ways to pluck the phosphate group.
>That was why I am trying to figure out if there are milder ways to pluck the phosphate group.
The mildest way is... just to wait. Phosphate groups are not very stable on proteins, and tend to fall off their amino acid. In vitro, depending on the conditions and micro-environment of the protein, a few days might be enough to lose a substantial amount of the phosphates. In your case, I don't think a non-specific dephosphorylation agent would be a good therapy, since phosphorylation events are important for a plethora of cellular functions. Maybe it would be better to specifically inhibit the relevant kinase, or boost (expression) of the specific phosphatase, if there is one (known).
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