I am very new on proteomics and I have formalin-fixed paraffin embedded (FFPE) tissue from temporomandibular joint of humans fetus and I'd like to perform a proteomics analysis with it. Does somone know some protocol that works well for this type of tissue?
Hello Filipe Santos
The biggest issue in trying to extract protein from FFPE samples lies in the nature of formaldehyde crosslinking. The real problem facing proteomic analysis of FFPE samples is either the difficulty in completely reversing these formaldehyde-induced modifications or the characterization of the chemical modifications that remain after sample treatment to break crosslinks. A deeper understanding of the mechanisms of cross-link reversal is needed.
Nevertheless, I have attached a few references below that may be helpful.
https://www.tandfonline.com/doi/abs/10.1080/14789450.2019.1615452?journalCode=ieru20
Article Analysis of formalin-fixed, paraffin-embedded (FFPE) tissue ...
Article Efficient extraction of proteins from formalin-fixed paraffi...
https://www.medscape.com/viewarticle/810682_7
Best.
Hi Filipe,
Here is a good publication which I followed and obtained results (protein identifications) from FFPE slides:
Anna Lie I did not know about this website. I already asked for a demostration. Thank so much.
Hi Malcolm Nobre
Thank you for the papers and also for the crosslinks mechanisms information. I did not know about it. I am going to study it better for sure. Thanks for your time.
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