I understand that low temperatures can affect spores of AMF, but there is any evidence or reference about isolating spores from soil samples that have been frozen for 6 months?
You can harvest AMF from Frozen soil sample.
Addy et al. (1998) showed that the infectivity of G. intraradices postfreezing was most effective when a low-temperature acclimation period was imposed prior to freezing.
Addy, H.D., Boswell, E.P., and Koide, R.T. 1998. Low temperature acclimation and freezing resistance of extraradical VA mycorrhizal hyphae. Mycol. Res. 102: 582–586.
Addy, H.D., Schaffer, G.F., Miller, M.H., and Peterson, R.L. 1994. Survival of the external mycelium of a VAM fungus in frozen soil over winter. Mycorrhiza, 5: 1–5.
The phenology of arbuscular mycorrhizal fungi was determined over one year in a community in southern Ontario, Canada by John N. Klironomos et. al. 2001. Five fungal species from the same community were used to inoculate five plant species, in all possible combinations, and were subjected to either a freezing treatment or a drought treatment after which new seedlings were transplanted into the treated pots. The percent colonization of roots of each plant species was measured as the difference in mean colonization from the control. Freezing reduced percent colonization in almost every case, whereas drought resulted in both increased and decreased percent colonization.
Can. J. Bot. 79: 1161–1166 (2001) DOI: 10.1139/cjb-79-10-1161
There are many reports on isolation of Mycorrhiza. Studies in Alpine , Tundra and Antarctica is also conducted.
A comprehensive study of Arctic plants from Spitsbergen was conducted by Hesselman (1900). Nespiak (1953) studied alpine plant roots in the Tatra Mountains. Katenin (1964) surveyed plants in Siberian Tundra. Miller and Laursen (1978) in Alaskan Arctic at Barrow and Miller (1982) on Eagle, Sunmmit, studiedthe mycorrhizal root of a variety of plants. Haselwandter and Read (1980) and Read and Haselwandter (1981) reported a variety of mycorrhizal types on roots of Alpine plants in Austrian Alps. Plants from Antarctic area were surveyed for mycorrhizae by Christie and Nicolson (1983) and Smith and Newton (1986).
The information on mycorrhizal status of arctic and alpine plants are available on many species. The result of the studies are not consistent. Alpine plants are mycorrhizal to a high degree (Haselwandter 1987) and similar results are reported for Arctic plants from Siberia (Katenin 1964) and from Alpine plants at Eagle Summit , Alaska by Miller (1982). On the other hand, Kohn and Stasovski (1990) and Vare et al (1992) described a remarkable paucity at least in the case of VA colonization in Arctic area.
Cross Reference
R. Treu, G. A. Laursen, S. L. Stephenson, J. C. Landolt, R. Densmore (1995) Mycorrhizae from Denali National Park and Preserve, Alaska. Mycorrhiza
Volume 6, Issue 1, pp 21–29
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