What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

When testing for outliers in a moderation analysis with a dichotomous moderator, is the overall sample size used to adjust Cook's and S.residuals?

This managed to throw me for a loop. When I'm assessing the presence of outliers before I conduct my moderation analysis I use SPSS to split the data file based upon the dichotomous moderator. I...

24 August 2020 6,578 3 View

Hello, does anyone have any links/resources for an extraction method of melatonin dissolved in AQ solution?

Hello, I currently have a product that has a small amount of melatonin dissolved in it (aqueous solution), and I need a sufficient amount to get a reading on an IR spec. I was thinking of...

04 December 2018 7,975 3 View

Does anyone have a protocol for the extraction of nuclear and cytoplasmic RNA from plant leaf tissue?

Hello, I'm looking for a protocol that will allow the extraction of nuclear and cytoplasmic RNA from the same plant leaf tissue sample (Arabidopsis thaliana). I have found protocols for the...

16 August 2018 158 2 View

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

10 October 2017 2,650 2 View

Does anyone know a good way of getting plasma from frozen whole blood?

11 July 2017 9,233 4 View

How to prevent background DIG staining in in situ PCR method?

I am performing in situ PCR of an RNA target in bovine lymph tissue; incorporating the DIG-dUTPs directly in the PCR product and then detection via anti-DIG Fab AP binding and subsequent...

08 February 2016 5,716 3 View

Are item means typically different in Xcalibre?

I am running an IRT analysis on an instrument in XCalibre, and the analysis reports substantially different means for the items than those calculated in Excel? Is there some weighting happening of...

30 January 2015 7,946 3 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

02 July 2014 7,096 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View