How to deprive ATP in cancer cell?

I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?

19 November 2019 5,972 1 View

Any suggestions for an accurate tool in bioinformatics to find pseudogene?

By giving a sequence as an input, are there any available computational tools for finding the pseudogenes in a particular organism?

05 June 2019 6,591 4 View

Trouble shooting allele specific pcr?

am setting up allele specific pcr for cyp21a2 gene that has a pseudogene. i have already amplified the normal gene with specific primers. using this as secondary product i am trying to set up...

31 December 2018 5,287 4 View

Is there anyone familiar with CYP21A2 gene?

Hello all, I am using panel sequancing and I found a homozygous mutstion in cyp21A2 gene. I did sanger but couldnt find this mutation. I think there is a possibility that I sequanced pseudogene....

09 October 2018 2,831 0 View

Does an alternate sequence in in-silico PCR mean presence of a pseudogene?

I tried designing primers for ELANE gene and came up with the following two sequences in in-silico PCR: >chr19:855442+856209 768 bp size >chr19_KI270868v1_alt:34328+35095 768 bp size. Both...

01 February 2018 9,010 3 View

Does an alternate sequence in in-silico PCR mean presence of a pseudogene?

I tried designing primers for ELANE gene and came up with the following two sequences in in-silico PCR: >chr19:855442+856209 768 bp size >chr19_KI270868v1_alt:34328+35095 768 bp size. Both...

31 January 2018 3,122 3 View

What should I do if my primers are amplifying two equal sequences (a gen and a pseodogene)?

10 November 2017 4,006 4 View

What should I do if my primers are amplifying two equal sequences (a gen and a pseodogene)?

06 November 2017 1,263 4 View

How to identify the Pseudogene in whole genome sequence ?

I have to identify the number of pseudo gene present in genome sequence, Please suggest me what are the methods by which i can identify the pseudogene sequences. Please suggest me some...

05 June 2017 6,777 3 View

Can you use the following methods to investigate these experimental situations?

1) Chromosome Translocation: chromosome microarray + FISH? + CGH? 2) Determination of the levels of gene expression of Gene XYZ with or without hormone treatment in a cell line: FISH +...

04 May 2017 6,208 1 View