I have the whole transcriptome of a non - model fish. I need to identify transcribed pseudogenes from it and do functional analysis of the pseudogenes. How to make the technical programme for this work? Is it possible?
I am looking for Parenting styles questionnaire for my research. Is there any validated questionnaire available in Malay language?
07 February 2022 4,791 2 View
I am using the accelerometer data obtained from the MPU6050 for position estimation. I have attached an image of the distance graphs obtained from the collected data when the object is not moving....
01 April 2019 2,771 6 View
I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !
04 July 2024 3,068 3 View
Hello, For my project I need to find the promoter region. I retrieved the sequence of my target pseudogene from NCBI and found several long ORFs. But now I am looking for a good tool to find...
09 July 2023 8,608 1 View
Hi I am currently working on a gene that has got many pseudogenes and is located in an MHC region. when I blast the primers I've designed (for qPCR), in the NCBI primer blast I get so many...
05 January 2022 6,515 3 View
I want to understand what kind of variations occur on long ncRNA. Like for example missense mutations, point mutations, frameshift mutations etc. Similarly, for pseudogenes what are the variant...
09 August 2020 4,513 1 View
Does anyone know if there is an established convention or rule on the minimum percent identity cutoff for nucleotide sequence in order to infer sequence homology? I am looking for pseudogenes...
30 March 2020 252 7 View
I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?
19 November 2019 6,168 1 View
Is q-PCR sensitive to Pseudogenes?
15 August 2019 3,004 2 View
By giving a sequence as an input, are there any available computational tools for finding the pseudogenes in a particular organism?
05 June 2019 6,690 4 View
am setting up allele specific pcr for cyp21a2 gene that has a pseudogene. i have already amplified the normal gene with specific primers. using this as secondary product i am trying to set up...
31 December 2018 5,339 4 View
Previously it was assumed that a nonsense mutation producing a premature stop codon on KRTHAP (relating to fur loss) was only 240 000 years old (Human type I hair keratin pseudogene φhHaA has...
15 December 2018 2,817 0 View