I would like to locate LSCs in cytometry files of patients that have been diagnosed using the Euroflow panels for ALL and AML. I am thinking of starting with the CD34+CD38-CD45dim phenotype, maybe also looking at CD123+ in AML and other potential markers for LSCs (also Lin-). However I am afraid I this population could present an overlap with other more immature cells, has anyone had experience with this before and could tell me how easy/difficult it is to separate this population from others? Maybe certain markers that should be negative in LSCs and might be positive in other possibly overlapping populations.
Thanks!
The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies. In my opinion, using combination of other examinations consist of: bonemarrow smear, Immunophenotyping, abnormal chromsome del(5q), abnormal gene (RUNX1, FLT3, NRAS, NPM1) to determine AML or ALL will provide more comprehensive results.
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