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Questions related from Davis Beltran
We would like to manage in an efficient way our newly conceived flow cytometry and and would like to find a good way to manage our bookings Please Help
22 April 2024 866 1 View
is it necessary to starve cells while using a dual luciferase assay to detect expression on TGF-b cell signaling? I will perform an experiment and I would like to know the pros and cons of...
02 October 2023 6,491 2 View
Hello Dear all! I would like to know if it is possible to have a stable cell line with a response element attached to a luciferase signal, also, Could it be possible to have in the same stable...
11 September 2023 2,693 2 View
I would like to transfect HEK293 with a plasmid containing a luciferase (downstream of a response element to this pathway) to check a cell signal pathway, but in order to have a population of...
21 July 2023 878 1 View
Hello dear all, at the moment I have a plasmid exhibiting 2 luciferase signals to monitor 2 cell signaling pathways. Since this Luciferase reporter was originally designed to create a stable...
14 July 2023 5,047 2 View
Hello there! Could anyone help me out?? I'm new to Luciferase assays; I would like to develop a dual luciferase assay using a d-Luciferin substrate luciferase as my reporter luciferase and a...
29 March 2023 5,527 0 View
I'm quite new to the cell signaling world, maybe it is a naive question but why would be beneficial to tag my sample with V5?
23 January 2023 560 1 View
Hello dear All! I had collected PBMCs from clinical patients and would like to seek for cytotoxicity activity of T cells with several intracellular markers by Flow Cytometry I had set up...
21 March 2022 5,045 2 View
I'm planning to collect blood in EDTA tubes in a remote area in which I don't have access to immediate staining of cells. I'll be collecting samples for 2 weeks then transporting cells to the lab...
13 October 2021 4,090 2 View
Soon i'll do acquisition of samples using cyTOF but i would like to have some hands on in how to do the analysis practicing with some files
17 November 2017 1,129 3 View
I'm trying to figure out with monoclonal ab anti human CD49a to get, i have to decide either from clones TS2/7 or SR84, i want to use it for FFPE tissues fro histochemistry staining! any ideas...
26 January 2017 1,879 0 View
Hello do you have any recommended MOI to infect Dendritic cells with dengue virus in vitro, i want to use the lowest as possible owe to my virus stock (it has a low PFU/ml) i wan to see if they...
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