Is it possible to get values of zero in the raw data obtained from a protein microarray?

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FX Reymond Sutandy

Hi Pauline,

If you mean no signal detected yes it is possible. Although this is really depend on the specificity of your detection. In most of the cases there always be a background signal that make the signal value > 0 therefore normalization with background signal removal is required.

Dejun Li

Yes, it is absolutely possible. The signal intensity is always related to some factors such as Laser Power, background signal, sample dilution, etc.