One RAPD primer gave linked marker with disease resistance in blackgram. The same primer is giving different marker in different F2 population for the same character. How is it possible?
Hey! As per the literature it is not possible to probe either normal or mutated allele accurately. The probe can detect sequence close to the gene of interest not exactly the gene. So we can not exactly diagnose the gene.
check the following links you may get some useful information.
https://www.ndsu.edu/pubweb/~mcclean/plsc431/markers/marker3.htm
http://www.uvm.edu/~cgep/Education/Marker.html
Testing for Association between Disease and Linked Marker Loci: A Log-linear-Model Analysis
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1683048/pdf/ajhg00089-0112.pdf
RAPD markers are randomly amplified DNA fragments, so, you CAN get two different DNA fragments associated with resistance in two different population.
The question is:
1) What is the distance between your RAPD markers and resistance traits? (how many F2 plants have you used for your study?)
2) Is it reproducible in different experiments? (different DNA isolations, different PCR kits, different resistance rating, F3 vs. F2 populations).
3) Can you convert the RAPD markers into SCARs or PCR-RFLPs? Does it still work?
Thank you Alex. The method used for the identification of linked marker was Bulked Segregant Analysis (BSA) and plants used in bulk were 10 in each bulk.
Darshan, it is too early to say that your RAPDs are linked to any trait.
At this stage any idea can be considered as possible.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases