We currently working on wolffish larvae hatched from wild-caught eggs and at ca 180 dph we're still not sure to which species they belong. Hopefully, we'll see this later in the juvenile/adult phase.
However, for similar, future work it would be extremely valuable if we can determine at the onset of the experiment, to which species the larvae belong.
Any information on genotyping/bar-coding procedures appreciated.
There are COI bar-codes for the species: http://www.boldsystems.org/index.php/Public_SearchTerms?query=Anarhichas[tax]
The systematists at BioEnv in Gothenburg likely know the details on how to do it.
Hi Thrandur
Yes I guess you could use COI barcoding. Put eggs / larval tissue pieces in individual tubes with ethanol. On Island Anna Kristín Daníelsdóttir at MATIS could perhaps help further.
http://www.matis.is/english/emphasis/food-safety-and-environment/genetics/
Hi Phil, and thanks. However, just out of curiosity, do you know of any company in Ireland which would be able to do COI barcoding on these fish? I've no idea how quick, expensive... etc such work is, so perhaps it's good to shop around?!
Another and possibly cheaper and faster option would be to develop species-specific primers, and run the PCR-product on a gel to see which of the primers worked, an thereby identify the samples. Then one can start with the samples in the morning a nd get the answer the same afternoon.
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